Based on the current state and the evolution of the new crown epidemic in China, this pa-per uses machine learning models and historical data to predict the changes in the number of new crown infections in China in the next four months. First, analyzing the background and current situation of the new crown epidemic, and identified the research question by collecting relevant historical data, including indicators such as the number of infected people, the number of cured people, and the number of deaths. Second, employing ma-chine learning models and MIR model to predict the trend and scale of the number of new crown infections in China over the next four months. Finally, coming to a forecast conclu-sion: in the next four months, the number of new crown infections in China will drop very slightly (almost remain unchanged) every month, and the monthly infection rate will re-main at a low level. At the same time, discussing and summarizing the application value of the conclusions. The research results of this paper can provide useful references and guidance for government policymakers and the public, helping them better deal with the epidemic and formulate corresponding measures. In addition, the research methods and models in this paper also have a certain degree of versatility, which can provide a certain reference for other countries and regions to predict the trend and scale of the new crown epidemic.

Food is essential for human survival. Dietary nutrients contribute significantly to our health. As a typical group that engage in a great deal of physical activity, athletes require a diet rich in the right kinds of nutrients in order to keep their bodies running at peak efficiency. Basketball is an intensely competitive sport. To achieve victory, both teams will engage in physical and technical confrontation. Contemporary basketball is distinguished by its intense rivalry. As basketball evolves and strives to reach a higher level, the physical requirements for athletes become increasingly stringent. Diet and nutrition are the foundation for athletes. A nutritious diet is a formula for success. Athletes should consume the appropriate amount of energy required by the body. This paper examines the physiological characteristics and nutritional requirements of athletes through a literature review. In-depth research will be conducted to determine the nutritional requirements of basketball players and make dietary recommendations. The purpose of this study is to make dietary recommendations for basketball players by analysing their fundamental signs and nutritional requirements.

Gastric cancer (GC) is one of the most common cancers in worldwide range which ranks fifth, and it is also third most common cancerous death reason. Although there has been a decline in the rate of incidence and mortality over recent fifty years, cure for GC is extremely hard to achieve. The symptoms are not conspicuous at early stage of the GC patients usually, which is the reason GC is diagnosed at terminal stage. And it is worse that the prognosis effect is outrageously bad, the median survival times are mostly less than a year. Under this condition, prevention is the most efficient method to reduce the incidence and mortality, meanwhile, the application of newly emerged therapy should be attached importance to GC. To create accurate treatment to achieve this goal, it is necessary to comprehend the mechanism of the carcinogenesis, and mechanism of cancer development, the critical transformation points derived from those mechanism will divide the different methodology applying period. This article mainly focuses on the cancer stem cell (CSC) aspect of carcinogenesis, cancer development to inform the points which can be utilized to intervene and new therapy method.

Due to the quite unhealthy diet that people normally have in recent years, obesity becomes a more and more popular health issue. In recent years, it even becomes a risk factor for deaths. This essay mainly focuses on how the components in diet may cause obesity and other diseases and what components are required to make up of a healthy and balanced diet. To figure out a possible solution for the raising negative consequences brought by obesity, like coronary heart disease and type II diabetes, some investigations have been done. The researchers provided mice with either a diet high in fat or a normal diet to simulate the healthy diet and a quite unhealthy one that humans may take. Some extra tests like glucose tests are taken to show whether the mice have got the symptoms of diabetes. The simulation lasted for two months, an overall higher weight for the mice that were fed with high-fat diet was seen, which indicates that high-fat diet will put more weight on creatures. Also, high-fat diet overall leads to a higher glucose level, which suggests that unhealthy diet may show a decrease in the ability to decompose glucose and therefore causes diabetes. After the data is obtained and the result was drawn, nutritionists may cooperate with doctors and try to figure out the best possible diet for the patients that have very high risk of getting or already suffering from obesity, CHD or type 2 diabetes.

Cancer has a high mortality and prevalence rate in the world. CRISPR-Cas9 is one of the novel and most common gene-editing techniques. Compared with the first two generations of gene-editing technologies, CRISPR-Cas9 system has the advantages of easy design, low cost, high efficiency and so on. sgRNA guides Cas9 to the site of the targeted gene, and Cas9 cuts the DNA strand at that site, triggering the NHEJ or HDR mechanism so as to achieve the purpose of deletion or insertion. CRISPR-Cas9 can be combined with other factors for other purposes, such as CRISPRa, CRISPRi, and base editing. The CRISPR system now has been used extensively for research into biological mechanisms and disease treatments. Since cancer is controlled by genes, a number of researchers in recent years have looked at using the CRISPR system to treat cancer. The CRISPR technology has greatly improved our understanding of cancer and the factors that affect it, and has had a major impact on the study and treatment of cancer. CRISPR gene editing can quickly and efficiently generate gene knockouts and regulate gene expression to identify relevant genes that influence cancer growth. This review systematically introduces CRISPR-Cas9 and its application methods, delivery modes, and discusses some studies using cell lines and organoids in vitro and animal models for cancer therapy in vivo.

Hand, foot, and mouth disease (HFMD) is an infectious disease caused by the intestinal RNA virus. Hand, foot, and mouth disease can occur every season, with no significant difference in specificity. However, the more frequent season of onset is spring and summer, with April to September as the main month of onset. Coastal areas are relatively popular areas of incidence. HFMD is mainly characterized by herpes with different sizes and clinical manifestations, such as in the hands, feet, and mouth. This article categorizes drugs into two categories: antiviral drugs and antiviral prevention. In describing antiviral drugs, they are classified them into traditional Chinese medicine and non-traditional Chinese medicine. At the same time, the two most effective and commonly used drugs or methods are summarized for preventing HFMD, namely the EV-71 virus vaccine for HFMD, and the washable hand sanitizer that prevents HFMD by blocking the transmission path of the virus. A comprehensive description and summary of the current treatment methods and drugs for combating and treating HFMD. This article provides a comprehensive description of different drugs by elaborating on their advantages and therapeutic principles, combined with their related pharmacological effects.

14-3-3 protein is a dimer protein extracted from bovine brain cells, a highly conserved protein prevalent in eukaryotes. There are seven subtypes in mammals, respectively σ, ζ, β, γ, η, ε and τ. The subtypes differ in content and function. It was found that the protein interacts with corresponding ligand protein in regulating life activities, such as: cell cycle regulation, cell development, the transmission of cell signal molecules plays an important role. At the same time, related regulators have been developed based on cell-cell interaction. In recent years, the exploration of 14-3-3 protein has been deepening gradually. Inhibition or stabilizers of the 14-3-3 protein in question have been studied or discovered, and have become a new target for treating diseases. This paper reviews the effects of 14-3-3 protein on neurological disease, cardiovascular and cerebrovascular diseases and cancer are reviewed. It is found that protein subtypes affect the occurrence of diseases.

Cancer immunotherapy has been a hot topic of cancer therapy discussion for over decades. Several successful cancer immunotherapies have already existed for about 30 years, however it is just in the past decade that immunotherapy has achieved broad breakthrough on patient survival in multiple high-incidence cancer indications. Immunotherapy, as a promising therapy depending mainly on the mechanism that immune cells work to eliminate cancer cells, has three hot topics recently. Cancer vaccine is a therapeutic vaccine that typically involves exogenous administration of selected tumour antigens to activate dendritic cells (DCs), or even DCs themselves in order to initiate and stimulate immune response to tumour cells, regain their control over tumour growth, induce existed tumour regression and eradicate minimal residual disease. Chimeric antigen receptor (CAR) T-cell therapy uses a patient's own T cells, but genetically engineered to express a synthetic receptor that binds to a tumour antigen more precisely and efficiently, to serve as more effective army against tumours. Immune checkpoint blockade (ICB) depends on blocking certain receptors and their ligands involved in pathways that attenuate T cell activation — for example, cytotoxic T lymphocyte antigen 4 (CTLA4), programmed cell death 1 (PD1) and its ligand, PDL1 — to restore T cells’ activity and prevent acquired peripheral tolerance to tumour antigens. This review gives a brief introduction to how human immune system works and a basic overview on the principles of cancer vaccine, chimeric antigen receptor (CAR) T-cell and immune checkpoint blockade (ICB).

The rabies virus, which mostly originates from the bite of a sick dog or cat and spreads, kills tens of thousands of people each year, and it is super lethal, with almost no one surviving the infection, s so, everyone is afraid of it. The main body of the rabies virus is bullet-shaped, and each combination of proteins in it is an innate destroyer. From the destruction of cells by the G and M proteins to the transcription of the P and L proteins in concert with each other, the precision, and division of labor of the rabies virus are obvious. Of these, the L protein is the key to the operation of everything, and this paper will explore the destruction of each protein, thereby stopping the spread of RABV. In particular, the possibility that the L protein, unlike rabies vaccines currently on the market, might be a future drug design idea by destroying the structure of RABV after the virus enters the cell. Keywords: Rabies, RABV, Protein Matching, L protein.

Hepatitis B virus core protein (HBV Cp) is closely involved in the viral assembly, nuclear functions, compartment for reverse transcription, and intra-cellular trafficking. Therefore, modulation of HBV Cp assembly is a promising method to control HBV infection in both preclinical and clinical studies. In this paper, two ligands of HAP18 and AT-130, as representations of heteroaryldihydropyrimidines and phenylpropenamides, have been chosen. Their Binding modes and conformations docking with the HBV Cp have been simulated by a Monte Carlo method. Strong polar contact in HAP18-bound and relatively weak interaction dominated by the Lennard-Jones potential in AT130-bound have been observed, respectively. Although different binding modes result in different assembly behaviors of HBV core protein, both of them strongly influence the quaternary structure of the Cp assembly, changing the spatial relationship between dimers, and inducing noninfective Cp misassembly. The simulation is expected to be helpful to get some insight into the antiviral mechanism of HBV Cp assembly modulation.